Antibacterial Activity of Facial Wash Formulated with Pegagang (Centella asiatica) and Sweet Orange (Citrus sinensis) Extracts against Staphylococcus aureus and Propionibacterium Acnes

Abstract

Acne is a common skin disorder caused by Staphylococcus aureus and Propionibacterium acnes, and is influenced by hormonal, sebum, and lifestyle factors. One of the preventive measures can be the use of facial washes made from natural extracts. This study aimed to determine the most effective concentration of a facial wash combining Pegagang (Centella asiatica) and Sweet Orange (Citrus sinensis) extracts in inhibiting the growth of acne-causing bacteria. The disc diffusion method was used in this studyThe facial wash formulations were prepared in three variations of Pegagang extract concentrations (6%, 12%, and 15%) with a fixed Sweet Orange extract concentration of 10%. The F3 formula showed the highest antibacterial activity, with an average inhibition diameter of 22.74 mm, and was not significantly different from the positive control. These findings suggest that Formula F3 is the most effective and has strong potential to be developed as a natural-based facial wash for acne prevention.